The assay of the enzymatic activity of alkaline phosphahatase was based on the spectrophotometric detection of a colored compound, p-nitrophenol, monitored at 405 nm, which is the product of the catalytic decomposition of a colourless substrate.
Since the alkaline phosphatase is a metalloprotein, it was possible to preconcentrate the enzyme in-line using a NTA Superflow resin charged with Zn(II) ions. The developed sequential injection method allowed the enzymatic assay to be carried out within 0.044–0.441 unit per mL of enzyme activity in samples of natural waters.
Determination of Alkaline Phosphatase
by SI Based Sorbent Extaction
2.3.25.
When compared to the batch, manual diethanolamine assay, the incubation time was reduced 25 times resulting in sampling rate of 17 s/hour. Also, SI methodology consumes far less reagents and is less laborious than manual technique. When compared to the previously published FIA methods, SI methodology allows considerable reduction of the incubation time , higher sampling rate lesser reagent consumption.
